RT007AMV Reverse Transcriptase, 1000U. (Kit)
Product code: MX-13942
RT007 AMV. reverse transcriptase was isolated from Avian Myeloblastosis Virus ( AMV) as the holoenzyme of molecular weight 157,000 Daltons, using a modification of the method described by Houts etc. This preparation is free of nuclease.
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Product description
RT007 AMV. reverse transcriptase was isolated from Avian Myeloblastosis Virus ( AMV) as the holoenzyme of molecular weight 157,000 Daltons, using a modification of the method described by Houts etc. This preparation is free of nuclease. It is qualified for cDNA synthesis and also for dideoxy sequencing of DNA and RNA Total RNA or poly A+ RNA can be used as template, and optimal temperature is 42-55'C, up to 60°C. If NaPPi Is used, 37~41'C is preferred. RT reaction buffer covered by patents can be used either as 5X /10X stock depending on different purpose (details in standard application). Up to 10-12kb cDNA may be obtained according to standard application.
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